20 回分 pHST-1 Vector, linearized
Annealing Buffer Ligase Mixture pHST Reverse Sequence Primer
pHST Forward Sequence Primer Competent Cell JM109 (50 μl × 20) SOC Medium (0.5 ml × 20)
The effect on cloning using different amounts of PCR fragments (about 1kb) was tested regarding the DynaExpress Hetero-Stagger PCR Cloning Kit and a TA cloning Kit (Supplier A).
The recommended protocol from each supplier was followed. After the reaction between PCR fragments and vector, competent cells were transformed with 1/3 amount of reaction mixture.
Half of the amounts of the cells were plated onto the agar plate. The white bars and the purple bars show the numbers of white colonies. The blue bars show the numbers of blue colonies.